Explanation

To evaluate the expression 2^2n + 1, we need to follow the order of operations (PEMDAS):

Calculate the exponent: 2^2n = 2^(2*3) = 2^6

Calculate the value of 2^6: 2^6 = 64

Add 1 to the result: 64 + 1 = 65

Therefore, the correct answer is: 65